This is the home page for the Neuroscience Program's Sliding Microtome, housed in the Van Hooser Lab (Bassine 327).
For safety's sake, it is important to first be trained by someone who knows how to use it.
The manual for the freezing stage is online here.
The microtome is used for histology - slicing frozen brain tissue for later staining and imaging.
The microtome was provided by the Neuroscience Program training grant and is therefore a program resource for all neuroscience labs, maintained by VH Lab, room 327. Please refer to the manual to familiarize yourself with the piece of equipment and refer back to if you have any questions.
First, if you:
- haven’t signed up for the microtome Google calendar
- aren’t on the mailing list
- or haven’t been trained
Then you absolutely should not be using the microtome! Seriously, I promise that you would much rather postpone your experiment now than be responsible for the inevitable (and expensive) damage later. This is not something you can teach yourself!
- If there is an open bottle of Tissue-Tek above the microtome, use it.
- If you finish a bottle of Tissue-Tek, go get a new one from YOUR OWN LAB'S stock.
- If you take the last bottle of your own lab's stock, go tell YOUR OWN LAB to order more.
(That's currently Nora/order page for Van Hooser).
- When new bottles come in, LABEL THEM.
You will need dry ice to use the microtome. Where would I get dry ice you may ask; Rosensteil 2nd floor. Bring a bucket to carry the ice back in. Weigh out what you want, then write down how much you took, your name, and the lab name.
We have a mallet and fabric bag to smash dry ice if needed. Fun Fact! The mallet is named after Thor's hammer. We have fabric bags in the microtome area. They're drawstring and everything. We also have an alcohol sprayer, KimWipes, and a drawer organizer containing brushes for moving slices, and pencils for marking slides. It's almost as if we planned it that way!
- Point people